Application of Serum Bottles in Cell Culture Media
Serum bottles are specialized containers for storing serum, typically made of PET material through an injection stretch blow molding (ISBM) process. Cell culture media, essential for cell growth, can be classified into natural media and synthetic media, both of which can be stored in serum bottles.
Serum bottles are specialized containers for storing serum, typically made of PET material through an injection stretch blow molding (ISBM) process. Cell culture media, essential for cell growth, can be classified into natural media and synthetic media, both of which can be stored in serum bottles.
Natural Culture Media
The most commonly used natural culture medium is serum, with calf serum being the most widely utilized. Serum contains multiple cell growth factors, adhesion-promoting factors, and various bioactive substances that, when combined with synthetic media, enhance cell proliferation and attachment. To maintain its stability, serum must be stored in specialized serum bottles at -5°C to -20°C.
Synthetic Culture Media
Synthetic media are formulated based on the precise requirements of different cell types, containing carbohydrates, amino acids, lipids, inorganic salts, vitamins, trace elements, and cell growth factors. The defined composition allows for better experimental control. However, certain unknown natural components in serum cannot yet be replaced by synthetic substitutes, making it necessary to supplement synthetic media with natural components. The most common practice is adding calf serum to overcome the limitations of synthetic media.
Advantages of Serum Bottles for Storage
Square Shape: Easy to grip and handle.
Chemical Resistance: Resistant to most acids and bases, ensuring compatibility with various culture media.
Low Temperature Durability: Brittle temperature at -70°C, maintaining a degree of flexibility even at -30°C.
With their excellent chemical stability, durability, and usability, serum bottles are ideal for storing both natural and synthetic culture media, ensuring the safety and effectiveness of cell culture processes.